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Finally, dimensionality reduction visualization was conducted via principal component analysis (PCA) on data before and after correction to assess the efficacy of batch correction and the discriminative power of sample grouping, ensuring the quality of data integration met the requirements for subsequent co-expression analysis

However, this effect was not reproduced in vivo: in the ileum and colon of metformin-treated mice, GLUT1 expression was markedly increased, whereas ATF4 levels remained unchanged (Supplementary Fig