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Many immunomodulatory factors are found in the tegument, the multi-layered outer surface of the parasite which is capable of quick self-renewal after damage, a process mediated by a population of somatic stem cells with a propensity to differentiate into tegument associated cells (147).The factors within the tegument have been extensively examined (148, 149)

Synergistic Natural Products in Anti-Ageing: Mechanistic Insights, Experimental Evidence, and Translational Perspectives Yuanze Sun 1,2 Lihong Sun 2 Johnson Liu 3 Ji He 4 Yu Feng 4 Jun Lu 1,4,5,* Received: 18 November 2025 Revised: 13 January 2026 Accepted: 20 January 2026 Published: 26 January 2026 2026 The authors

L-PAM as a single agent was highly active against MM.1S, KMS-12-PE, MOLP-2 and NCI-H929, inducing 2 logs of cell kills at the maximum dose (50 M)

Cell viability was determined by mixing an aliquot of the suspension 1:2 with trypan blue and counting viable cells using a hemocytometer

After permeabilization and blocking, primary antibody was added and incubated for 1 h at room temperature