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A80-229AP, Bethyl Laboratories, Inc.) in PBS/T/M was added for 1 h at room temperature, followed by wash and detection with 1 mg/mL (100 L/well) of p -Nitrophenyl Phosphate Substrate (Sigma-Aldrich) dissolved in diethanolamine buffer (pH 9.8) (Sigma-Aldrich)

Experimental design considerations include the importance of timing senolytic administration relative to senescence induction or disease progression, as the biological context affects both efficacy and interpretation of results

CJC-1295 + Ipamorelin (Injectable) CJC-1295 is a peptide analog of growth hormone releasing hormone (GHRH), and Ipamorelin is a peptide that mimics ghrelin (a hunger hormone) to trigger growth hormone release