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Vitamin B12 is an essential nutrient that plays a key role in energy production, brain function, and red blood cell formation

Upon binding to an uncharged tRNA, GCN2 undergoes a conformational change and is activated, resulting in inhibitory phosphorylation of eukaryotic translation initiation factor 2 (eIF2), preventing translation initiation under amino acid deficiency (Figure 1) (Berlanga et al., 1999

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