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3.6 mM KCl, 135 mM NaCl, 2 mM NaHCO 3 , 0.5 mM NaH 2 PO 4 , 1.5 mM CaCl 2 , 0.5 mM MgSO 4 , 10 mM HEPES, pH 7.4, adjusted with NaOH, and 0.2% BSA), containing varying concentrations of glucose for 1 h