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SDS PAGE Commercial protein samples have been analyzed by SDS PAGE performed by the Mini-PROTEAN Tetra System (BioRad) with Laemmli gel (10%T/5%C) consisting of separation and comb gel

We also now know that the cytosolic disulfide reducing power required for RNR activity and cell homeostasis can be delivered to its targets by either Trx1 in the absence of GSH or by GSH in the absence of Trx1 [108, 178]