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Total absence of functional acid labile subunit, resulting in severe insulin-like growth factor deficiency and moderate growth failure

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Multiple sources: Lott, McCown & Ross 2000, pp

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Cells (5 10 4 cells/well) in RPMI-1640 medium were plated in a flat-bottom 96-well microplate and treated with 20 L of different concentrations of the LAB supernatants for 48 h at 37 C in a humidified 5% CO 2 atmosphere, except for the 20% v/v concentration, which was treated with 40 L of the tested sample over 160 L of the cells