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The expression of these proteins was detected using a MACH 4 Universal HRP Polymer detection kit (Biocare Medical) and 3,39-diaminobenzidine (DAB substrate kit, Vector Laboratories, Burlingame, CA)

The cells appeared ordered with the organelles appearing almost normal under a transmission electron microscope with the collagen fibers arranged relatively regularly as compared to the model control group where the collagen fibers were irregular and disordered with the organelles being malformed (Wang et al., 2016)