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Before each experiment, cells were detached by trypsinization, counted using a hemocytometer, seeded in 24 well plates at 10,000 cells per well and incubated for 24 hrs prior to the initiation of each experiment

Proper storage and handling help reduce degradation processes such as hydrolysis, oxidation, and peptide aggregation, which can affect experimental reliability

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Examine the powder for discoloration (yellowing or browning), clumping, or stickiness