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The healing was effective, too: it preserved the function and biomechanics of the joint

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for 6 min, following which the reaction was terminated by the addition of a mixture of 10% trichloroacetic acid and 4% methanol, vortexing for 5 s, and centrifuging at 14,000 rpm for 2 min to precipitate the protein

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Sterile Water for Injection contains no preservatives and is strictly single-use the vial must be discarded after a single access