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After the polymerase chain reaction (PCR), its products were determined by 1% agarose gel electrophoresis, the target band was cut and purified, and the recovered products were used for Sanger sequencing

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The other described a patient with steroid refractory CD who showed no significant clinical or histologic response to 6 weeks of ED, necessitating a change in treatment and eventually responding to infliximab [34]

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