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Description

The gut microbiota of healthy adults belongs to Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria, of which Firmicutes and Bacteroidetes are dominant with a proportion of approximately 90% (Eckburg et al., 2005)

To quantify citrate and 2-OG, the culture supernatant was diluted 10-fold with methanol and transferred to a LC-2050C LT system (Shimadzu) connected to a LCMS-2050 system (Shimadzu)