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doi: 10.1371/journal.pone.0127558 49 KubotaMNakataRAdachiSWatanabeKHeikeTTakeshitaYet al

Vanin-1 activity is thought to counteract PPAR, as evidenced by the anti-inflammatory phenotypes of vanin-1-deficient mice that PPAR inhibitors inhibited (124)

Ring A, Le Lay S, Pohl J, Verkade P, Stremmel W

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GST-tagged TRABID NZF (40 g), 1 g of FLAG-tagged K48TUBE (LifeSensors, UM607), or 1 g of FLAG-tagged K63TUBE (LifeSensors, UM604) were added to cell lysates to capture K29-, K48-, or K63-linked chains, respectively, and were precipitated using Glutathione Sepharose (GE Healthcare, 17-0756-01) or anti-FLAG affinity resin