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For analysis, each urine sample was thawed at room temperatures and centrifuged at 3000 g for 10 min, followed by filtration of supernatant through a 0.22 m membrane Syringe Filter PTFE (AZ Chrom s.r.o., Bratislava, Slovak Republic)

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The most common effects are gastrointestinal and dose-dependent

To achieve this, these enzymes can bind non-substrate ligands (ligandin function) and/or catalyze the conjugation of glutathione onto the targeted molecules, the latter activity being exhibited by GSTs having a serine or a tyrosine as catalytic residues

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