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Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively

He plays with strong fundamentals, can score when needed, make plays for others, and holds his own defensively

Ethics declarations Ethics approval and consent to participate All animal experiments performed in this study were approved by the Institutional Animal Care and Use Committee of Anhui Medical University (approval number LLSC20231140)