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3g) A solution of 12 C-timonacic in PBS (final concentration 2.5 mM) was mixed with tenfold excess of 13 C-formaldehyde (final concentration 25 mM) and shaken at 37 C

Cell samples (5*10 6 cells) were collected and washed with PBS, followed by centrifugation at 800 g for 2 min, and the supernatant was discarded

There was no the significant difference in the level of ALT and AST among the different group when compared to control group, except for Group 3 (administration of color fruit juice for 12 hours) and Group 5 (administration of ketchup potato chips) at concentration of 30%

342 World Health Organization

Safety and efficacy of givinostat in boys with Duchenne muscular dystrophy (EPIDYS): a multicentre, randomised, double-blind, placebo-controlled, phase 3 trial