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Experimental conditions were optimized to maintain reaction linearity and to limit substrate conversion to 100-fold higher than cyclase activity ( + , a non-hydrolyzable NAD + analogue with enhanced GDPR-Cyclase activity

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The International Federations (IF) affected by this change were alerted sufficiently in advance in order to amend their rules and to put in place protocols to test for alcohol use and appropriately sanction athletes who do not abide by the rules of their sport

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