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They were cultured in an endothelial cell growth medium (#211-500, Cell Applications, United States), supplemented with antibiotics, and maintained at 37 C in a humidified incubator with 5% CO 2

Treatment with TMZ and PB each alone or in combination resulted in a significant decrease in IL-1 tissue level as compared to the untreated CIM group

Finally, the samples were blocked with the antifade mounting medium with DAPI

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