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(vi) Resuspend the cells in 1 ml of ice-cold extraction buffer (0.1% Triton-X and 0.6% sulfosalicylic acid in KPE) and homogenize cells with a Teflon pestle

Uncropped and unprocessed Western blot and gel images have been included in Supplementary Fig

showed that nutritionally induced oxidative stress in mice fed an antioxidant-deficient diet enhances the pathogenesis of coxsackievirus B3 infection and the development of myocarditis 135

Nat Catal

However, both aging and cancer are characterized by a series of partially overlapping hallmarks. The equivalence or antagonism between aging-associated deregulated nutrient-sensing and cancer-relevant alterations of cellular metabolism is complex