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577,578,579 Preclinical studies suggest that antibiotic therapy may reduce secondary bile acids associated with hepatic inflammation and metabolic HCC development, which can enhance anti-tumor immunity

doi: 10.1016/S0140-6736(14)60802-3 49 LiY-SLuoWZhuS-ALeiG-H

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Lineage-negative cells were enriched using the lineage cell depletion kit (Miltenyi Biotec, 130-110-470), differentiated in the presence of 20 ng ml 1 M-CSF (PeproTech, 315-02) for 3 days, treated with 10 g ml 1 -glucan or PBS for 24 h, cultured in the presence of M-CSF for 3 days, induced with 10 ng ml 1 LPS for 24 h before the medium was collected for quantification of cytokines