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This Gs-biased profile may contribute to sustained receptor signaling with less desensitization, though the clinical implications of signaling bias in GLP-1 therapeutics remain an area of active investigation

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Primary ESCs were seeded into 12-well plates and cultured until they reached 6080% confluency, followed by transfection with 20 pmol STAT6 siRNAs per well using the Lipofectamine RNAiMAX reagent (Invitrogen, California, USA) in Opti-MEM (Gibco, New York, USA) according to the manufacturers instructions