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Sirt1 activation prevents high glucose-induced angiotensin converting enzyme 2 downregulation in renal tubular cells by regulating the TIMP3/ADAM17 pathway

Yang X, Xu S, Qian Y, et al

10.1016/j.cell.2017.11.048 Cell 81 IshiiT.WarabiE.MannG

However, the Library Normalization step was omitted and normalization was instead performed by pooling equal amounts of libraries after Quant-iT PicoGreen quantification

Formula: Volume of Diluent (mL) = Actual Active Compound (mg) / Desired Concentration (mg/mL) Example: You have 99 mg of active 5-Amino-1MQ, and you want a final concentration of 10 mg/mL