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Animals under anesthesia were then transferred to a nose cone and continued to receive 15% isoflurane at an O 2 set to 1.0 L/min and then exsanguinated by cardiac puncture using a 25 gauge needle followed by cervical dislocation

171 Furthermore, H9C2 cardio myoblast cells were protected against glucose-induced stress by the methanol, ethanol, and aqueous seed extracts of Jamun

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