In vivo experiments in this study complied with the ARRIVE guidelines and were conducted in accordance with the National Research Councils Guide for the Care and Use of Laboratory Animals and approved by the Institutional Animal Care and Use Committee at Fudan University
Furthermore, the first three eigenvectors were collected from each MD simulation trajectory and plotted to demonstrate the residual displacement in the different conformations of the protein structure, where a gradient color change (from blue to white to red) specifies that there are regular leaps among the various conformation of protein structure throughout the trajectory (Fig
After incubation for 4 h at 37 C in 5% CO 2 humidified atmosphere, colony forming units were determined on Barratts minimal medium as described earlier
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