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The study was designed using three animals per stage and was meticulously observed for any signs of toxicity or death

The glutathione reductase inhibitor (GRI) 2-acetylamino-3-[4-(2-acetylamino-2-carboxyethylsulfanylthiocarbonylamino)phenylthiocarbamoylsulfanyl]propionic acid hydrate (2-AAPA) was purchased from Sigma-Aldrich

107 It has been suggested that selenium can regulate GPx4 expression through increasing ribosome density in downstream of UGA-Sec codons and sec incorporation efficiency in part by the degree of Sec-tRNA[Ser]SecUm34 methylation