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An OA mouse model was successfully established by injecting monosodium iodoacetate (MIA) in mouse knee joints 25 , specifically, involving anesthetic administration with 1.25% tribromoethanol (Bimake, M2920) at a dose of 0.2 mL/10 g body weight, followed by intra-articular injection of 10 L MIA (50 mg/ml, Sigma-Aldrich, 57858-25G-F) into the right knee joint using a 30-gauge needle

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Then, perform hand hygiene

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Lab studies show that nicotinamide doses above 20mM can lead to cell death