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The next day, the media was removed and 45K ECFCs per well in 50100 L of media was plated on top of the ADSC monolayer and incubated at 37C, 5% CO 2 for 36 hours before the addition of growth factors and inhibitors

For example, in mono-colonized mice, Bacteroides thetaiotaomicron can acquire sialic acid from mucosal polysaccharides and produce succinate, which subsequently serves as a carbon source for Clostridium difficile during infection (80)