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Description

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Respiratory parameters were calculated according to manufacturers instructions (Seahorse Bioscience)

International Journal of Surgical Investigation, 2 (1), 1-7

The Abl KD and SH2-KD proteins in pET21d vectors containing a C-terminal His 6 tag were each co-expressed with YopH phosphatase in TB medium and purification was performed as described previously using nickel-affinity chromatography (His-Trap FF crude, Cytiva) and anion exchange chromatography (Mono Q 5/50 GL, Cytiva) on an kta Avant system (Cytiva) 13,52