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Mol Cell Endocrinol 132:8191 Chin E, Zhou J, Bondy C (1992) Anatomical relationships in the patterns of insulin-like growth factor (IGF)-I, IGF binding protein-1, and IGF-I receptor gene expression in the rat kidney

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Hence we used an alternative approach where we analyzed the oxidized protein derivatized with Au 15 SG 12 -Ao after subsequent degradation of the grafted NC and trypsin digestion, whereby the putative oxime bond between the aminooxy on the NC and carbonylated amino acids on the protein remained intact