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Biological Role: Primarily studied for its role in healing and regeneration, especially in tissues like muscles, tendons, and the gastrointestinal tract

mTORC1 signaling induces genes involved in amino acid synthesis, transport, and tRNA charging through ATF4 activation To validate and expand the findings from the RNA-seq analysis, a Nanostring codeset was designed to simultaneously quantify transcripts of genes involved in the enriched processes above (see methods)

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Signalling activates PLC- DAG + IP3 intracellular Ca + PKC (via Gq) plus Gs-cAMP-PKA, with a balanced profile neither Gs- nor -arrestin-biased in recent cryo-EM and BRET studies (2020-2023)

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