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We used a human BBB co-culture model improved by cARLA treatment, as described in our paper [40], for the impedance, cellular uptake and permeability experiments

To induce intracellular GSH depletion, cells were incubated in Cys 2 -free medium or with BSO (1000 M) treatment or erastin (10 M) treatment

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Finally, the aqueous suspension of FA-CMC-GNA NPs was subjected to freeze-drying with a lyophilizer (Shel Lab, Cornelius, OR, USA) to yield a powdered form