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Introduced in the 1980s, organotypic slice cultures were traditionally cultured using the roller-tube method but have since been optimized to culture via the air-liquid interface method, which allows slices to simultaneously receive nutrients from the culture medium and humidified air, facilitating long-term viability (Ghwiler, 1981

Intracellular assembly of supramolecular peptide nanostructures controlled by visible light

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168 An update to the literature in 2022 further showed the efficacy of heparin and lidocaine combined for relieving pain and urgency symptoms associated with IC/BPS in two studies (prospective and randomized cross-over)