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In an aspect the disclosure provides the CRISPR-Cas system or CRISPR enzyme system wherein the modification comprises altering loops and/or hairpins

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Cytochemical SA--galactosidase assay Cells were seeded in six-well plates, fixed with 0.5% glutaraldehyde (Sigma, G5882) in PBS for 15 min, washed with 1 mM MgCl 2 /PBS (pH 6.0) and then incubated with X-Gal staining solution (1 mg ml 1 of X-Gal (Thermo Scientific, 2737087), 5 mM K 3 (Fe(CN) 6 ) and 5 mM K 4 (Fe(CN) 6 ) for 68 h at 37 C

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