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Studies have confirmed (121, 122) that p53 can induce ferroptosis not only by inhibiting SLC7A11 activity but also by increasing CDKN1A expression or decreasing DPP4 activity, suggesting the dual regulatory role of p53 in the regulation of ferroptosis

Viral vectors AAV.PHP.eB vectors were prepared at PackGene Biotechnology (Guangzhou, China) respectively with the transgene cassettes: CMV-driven mCherry (titer: 1 10 13 GC/mL), Gpx1- MYC (1 10 13 GC/mL) , Gpx4- HA (1.28 10 13 GC/mL), and HC-promoter ( Rbm24 -Eh3 enhancer following with Hsp68 mini-promoter sequences)-driven Cre (1 10 13 GC/mL)

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Reported benefits include improved lean body mass , fat loss , better sleep quality , higher energy levels , and even enhanced skin and collagen (due to increased GH/IGF-1)

For this reason we believe the most effective plasmid design should place Gpx closest to the promoter, where it has the highest probability of being fully transcribed by RNA polymerase, and GR furthest from the promoter, since high concentrations of GR appear to be less critical given its efficacy