In accordance with previous results (17), we observe a unique and highly unusual fold for CTL1 among SLC proteins, with 10 transmembrane helices (M1M10), a large extracellular domain bridging M1 with M2, and three shorter amphipathic helices (AH1-3) parallel to the membrane that connect M4M5, M6M7, and M8M9, respectively (Fig 4A)
( D ) N-terminal TDE0362 (N0362, 23204 aa) was constitutively expressed in yeast cells using TEF promoter (p426TEF)
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Kupffer cells may also be exposed to lower glutamine concentrations than observed in the plasma because their sinusoidal arrangement within the hepatic lobule does not allow all cells to come into direct contact with circulating blood
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