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Assessment of live-cell ATP production Fibroblasts were detached by 0.05% Trypsin-EDTA (Gibco, #25300054) treatment and pcDNA3.2-V5-mtLuc (Addgene, #219666) was introduced using Amaxa Nucleofector 2 (Lonza) and the Amaxa Human Dermal Fibroblast Nucleofector Kit (Lonza, VPD-1001) according to protocol

Although EBV proteins may play a role in the progression of ME/CFS (52), researchers have hypothesised that the virus has a third state termed the abortive/leaky/lytic replication, as has been suggested upon analysis of the EBV genome in T and NK cells of patients with chronic EBV infection (134136)