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RT-PCR was conducted in 20 L of reaction mixture containing cDNA, TaqMan primer pairs, and TaqMan universal PCR master mix (Applied Biosystems, Foster City, CA, USA)

Clinical Significance Selenium is an essential trace element, involved in the aetiology of a number of diseases

Cells were treated with 100 ng/ml tunicamycin for indicated time

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Release of neutrophil extracellular traps by neutrophils stimulated with antiphospholipid antibodies: A newly identified mechanism of thrombosis in the antiphospholipid syndrome