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After further culturing for 24 h, each well was washed, 1 mg mL 1 MTT solution (100 L) was added, and the cells were re-incubated for 4 h in the dark

Injections and infusions of it were permitted within Wada rules in 2014 provided the volume was below 50 millilitres every six hours

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Hydration stands as the strongest benefit with solid scientific backing