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While insect cells offer several advantages for recombinant production, including efficient post-translational modifications and folding While insect cells offer several advantages for recombinant production, including efficient post-translational modifications and folding (Palomares et al., 2021), which can impact the function of some recombinant proteins ( FDL -encoded -N -acetylglucosamines

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Specify common side effects and their frequency Common effects are usually mild and localized

Glutaredoxins GRXs are oxidoreductases belonging to the TRX family, which is largely involved in the control of protein redox state

Results Cystine deprivation leads to rapid death of TNBC cells by mitochondrial oxidative stress independent of GSH loss To investigate the dependence of BC cells on Cyss, we first examined the cytoplasmic Cys/Cyss content in TNBC (Hs 578T and MDA-MB-231) and non-TNBC cell lines (MCF7), cultured for 8 h in the absence of Cyss or in the presence of buthionine sulfoximine (BSO), an inhibitor of GCL (Fig