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Alterations in gut microbiota can disrupt neurotransmitter homeostasis, particularly affecting serotonin (5-HT), norepinephrine (NE), and dopamine (DA) systems that are dysregulated in PTSD (Zhou et al., 2020)

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Then, 4 l of reagent B and 20 l of trichloroacetic acid were simultaneously added

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Western immunoblotting was conducted to examine protein expression related to key signaling pathways and cellular stress responses