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L.HimelfarbM

Biochim Biophys Acta (1957) 24(3):6589.10.1016/0006-3002(57)90271-8 9 MosharovECranfordMRBanerjeeR

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Reactions were quenched with acetonitrile 0, 5, 15 and 30 min, centrifuged at 3220 g for 20 min, and supernatants were analyzed by LC-MS/MS in columns equilibrated in 90% solvent A (0.2% formic acid)-10% solvent (acetonitrile) at a flow rate of 0.9 mL/min, after which the concentration of solvent A was decreased to 10% and maintained for 1.4 min