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10.3389/fneur.2017.00301 183 TangL.LiuS.LiS.ChenY.XieB.ZhouJ

Using an engineered C-terminal cleaving naturally split DnaE intein from Nostoc punctiforme the inventors developed a split intein mediated ultra-rapid purification of tagless protein (SIRP) that enables the purification of tagless recombinant protein from E