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For instance, when peripheral tissues become inflamed, the related signals might be transmitted to the brain through these receptors on the neurons, and the brain could then respond to these signals via neural pathways, thus regulating the level of peripheral inflammation

On the following day, the cells were treated with 0.5% Triton X-100 for 10 min and incubated with anti-YTHDF1 primary antibody overnight at 4C