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In both binding modes, arbutin interacts with residues on the CuA and CuB sides, respectively

From the above it can readily be seen that if levels of the two functional analogues of vitamin B2, namely FMN and FAD are reduced the following will happen

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In addition, when TyrBm crystals were soaked with copper and HQ for 16 hours, the crystals turned brown, in comparison to crystals that were soaked with zinc that did not show a change in color (data not shown)