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For activity screening, we adapted a GSTO1 substrate assay that relies on the quantification of the reduction of S -(4-nitrophenacyl)glutathione (4-NPG), a GSTO1-specific substrate 17 , using recombinant human GSTO1 in a high-throughput screening format

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Mitochondrial membrane potential (m) assay The m was measured as previously described [39]

Concepts Magn Reson Part A: Educational J 30:4064 Terpstra M, Henry PG, Gruetter R (2003) Measurement of reduced glutathione (GSH) in human brain using LCModel analysis of difference-edited spectra

Oxidative stress-part of the solution or part of the problem in the hypoxic environment of a brain tumor