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Moreover, cell viability was assessed by CCK8 and cell cycle was analyzed by flow cytometry-based PI staining

In fish, chronic stress has been reported to reduce nutrient utilization (Ring and Olsen, 1999

Biotechnol Prog 28:299311 Morelli E, Scarano G (2001) Synthesis and stability of phytochelatins induced by cadmium and lead in the marine diatom Phaeodactylum tricornutum