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One microgram of total RNA from each sample was reverse transcribed, and the real-time RT-PCR measurements were performed, as described previously [49], using an apparatus Aria MX (Agilent Technologies, Santa Clara, CA, USA) with the corresponding SYBR Green kit, according to the Promega manufacturers recommendations

A.MadejJ.et al (2024)

81 , who reported that lower blood HDL and greater LDL particle content were independently linked to NAFLD

Biochemical tests for AGHD diagnosis are imperfect, and their accuracy is strongly affected by the pre-test probability of the condition

Glutamine metabolism in the proliferation of GS-expression pituitary tumor cells