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The cells were harvested by centrifugation (1000 x g , 2 min) and resuspended in lysis buffer (5% w/v 5-sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA (KPE buffer [35])) together with 425600 m-diameter acid-washed glass beads (Sigma) at a buffer/bead ratio of 1:2 by volume

Targeting such metabolic programmes presents novel opportunities to enhance drug responses and improve patient outcomes 9

It also supports detoxification processes which contribute to better overall health and immune system function

Conversely, many everyday medications are potent inhibitors of these same enzymes, creating the potential for complex, bidirectional interactions

doi: 10.1056/NEJMoa2116846 105 GouldR