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Reconstitution with Care: Always use Bacteriostatic Reconstitution Water (bac)

Kumar A, Singh CK, Lavoie HA, Dipette DJ, Singh US

[DOI] [PMC free article] [PubMed] [Google Scholar] 298

Singularized cells from COs were seed at a density of 50,000 cells/well in 100L Hanks Balanced Salt Solution (HBSS) buffer in a 96-well white polystyrene plate (Greiner CELLSTAR, 655083)

In contrast, non-specific responses, such as those observed by Zernecke et al., do not settle the argument either way: they might be evidence against discrete signatures, but could also arise simply because of interdependence between different emotional states