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In South Asia, Leishmania donovani shows high resistance to sodium stibogluconate (SSG) and declining susceptibility to miltefosine, necessitating alternatives like liposomal amphotericin B (L-AmB) and combination therapies

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After 1.5 g of residue was dissolved in 10 mL of methanol, 0.15 mL of hydrazine monohydrate was added, and this mixture was refluxed at 80C for 1 h

Ethics statement The experiments reported in this manuscript were conducted according to the guidelines stated in the Principles of Laboratory Animal Care ( Author contributions CV-F designed the study, performed the experiments, and prepared the manuscript draft

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